Table 1

Two-dimensional echocardiography views, acquisition techniques, and subjects to be assessed in patients after heart transplant"

Parasternal long-axis of ascending aorta		Parasternal long-axis of pulmonary artery with bifurcation
Image acquisition still frame + cine loop (5 beats)	Acquisition technique 2D+Color Doppler Subjects to assess and parameters to measure -internal diameter of proximal and distal AAO and suture line -presence of AAO suture stenosis or leakage -AV morphology and function	Image acquisition still frame + cine (5 beats)	Acquisition technique 2D+Color Doppler+PW+CW Subjects to assess and parameters to measure -internal diameter of proximal and distal PA and suture line -presence of PA suture stenosis or leakage -PV morphology and function (anterograde and retrograde flow velocity)
Parasternal long-axis of LV		Parasternal short-axis of LV at MV level
Image acquisition still frame + cine (5 beats)	Acquisition technique 2D+M mode+Color Doppler Subjects to assess and parameters to measure -LV size, function, and wall thickness -LA morphology and size -AV and MV morphology and function -pericardial effusion	Image acquisition still frame + cine (5 beats)	Acquisition technique 2D+M mode Subjects to assess and parameters to measure -MV morphology and function -LV wall thickness at basal level -LV systolic function (basal segments) -pericardial effusion
Parasternal long-axis of RV inflow		Parasternal short-axis of LV at papillary muscle level
Image acquisition still frame + cine (5 beats)	Acquisition techniques 2D+Color Doppler +CW Subjects to assess and parameters to measure -TV morphology and function (anterograde and retrograde flow velocity) -RA morphology	Image acquisition still frame + cine (5 beats)	Acquisition techniques 2D+M mode Subjects to assess and parameters to measure -LV wall thickness at mid-level -LV systolic function (mid segments) -pericardial effusion
Parasternal axis of RV outflow tract		Parasternal short-axis of LV at apical level
Image acquisition still frame + cine (5 beats)	Acquisition techniques 2D+Color Doppler + PW+ CW Subjects to assess and parameters to measure -RVOT internal diameter and anterograde flow velocity -PV morphology and function -AV morphology and function -presence of ASD and VSD	Image acquisition still frame + cine (5 beats)	Acquisition technique 2D Subjects to assess and parameters to measure -LV wall thickness at apical level -LV systolic function (apical segments) -pericardial effusion
Parasternal short-axis at aortic valve level		Apical four chamber
Image acquisition still frame + cine (5 beats)	Acquisition techniques 2D+Color Doppler+CW Subjects to assess and parameters to measure -TV morphology and function -AV morphology and function -presence of ASD and VSD	Image acquisition still frame + cine (5 beats)	Acquisition technique 2D+Color Doppler+PW+CW Subjects to assess and parameters to measure -suture, atrial and ventricular size -MV+TV morphology and function -pericardial effusion
LV focused apical 4-chamber		Apical five-chamber
Image acquisition Cine (5 beats)	Acquisition techniques 2D Subjects to assess and parameters to measure -LV size, wall motion and function -presence of VSD -pericardial perfusion	Image acquisition Still image + cine (5 beats)	Acquisition techniques 2D+Color Doppler+PW+CW Subjects to assess and parameters to measure -AV morphology and function -LVOT morphology and anterograde flow
Apical 3-chamber		RV focused apical four chamber
Image acquisition Still image + cine (5 beats)	Acquisition techniques 2D+Color Doppler Subjects to assess and parameters to measure -LA and LV morphology -LV wall motion and function -MV morphology and function -AV morphology and function -pericardial perfusion	Image acquisition Still image + cine (5 beats)	Acquisition techniques 2D Subjects to assess and parameters to measure -RV morphology and size -RV wall motion and function -pericardial perfusion
LV focused apical 3-chamber		TAPSE
Image acquisition Cine (5 beats)	Acquisition techniques 2D Subjects to assess and parameters to measure -LV morphology -LV wall motion and function -pericardial perfusion	Image acquisition Still image	Acquisition techniques M mode Subjects to assess and parameters to measure Tricuspid annular plane systolic excursion (TAPSE)
Apical 2-chamber		Subcostal apical 4-chamber
Image acquisition Still image + cine (5 beats)	Acquisition techniques 2D+Color Doppler+CW Subjects to assess and parameters to measure -LA and LV morphology -LV wall motion and function -MV morphology and function -pericardial perfusion	Image acquisition Still image + cine (5 beats)	Acquisition techniques 2D+Color Doppler Subjects to assess and parameters to measure -presence of ASD -RV free wall thickness -pericardial perfusion
LV focused apical 2-chamber		Subcostal long axis of inferior vena cava
Image acquisition Cine (5 beats)	Acquisition techniques 2D Subjects to assess and parameters to measure -LV morphology -LV wall motion and function	Image acquisition Still image + cine (5 beats)	Acquisition techniques 2D+M mode Subjects to assess and parameters to measure -IVC proximal diameter and IVC collapse -estimated RA pressure
Subcostal bi-atrial view		Supraclavicular aortic arch long axis
Image acquisition Still image + cine (5 beats)	Acquisition techniques 2D+Color Doppler Subjects to assess and parameters to measure -presence of ASD -SVC+IVC flow	Image acquisition Still image + cine (5 beats)	Acquisition techniques 2D+Color Doppler Subjects to assess and parameters to measure -aorta morphology, diameters and flow

Table 2

Spectral and tissue Doppler echocardiography views, acquisition techniques, and subjects to be assessed in patients after heart transplant"

Mitral anterograde flow		LVOT anterograde flow
	Acquisition techniques PW Subjects to assess and parameters to measure -MV E wave velocity, A wave velocity, and E/A ratio -E wave deceleration time		Acquisition techniques PW Subjects to assess and parameters to measure LVOT anterograde flow
Tricuspid anterograde flow		Pulmonary vein flow
	Acquisition techniques PW Subjects to assess and parameters to measure TV E wave velocity, A wave velocity, and E/A ratio		Acquisition techniques PW Subjects to assess and parameters to measure Ar wave peak velocity and duration S and D wave peak velocity
Pulmonary anterograde flow		Septal mitral annulus TDI
	Acquisition techniques PW Subjects to assess and parameters to measure PV anterograde flow velocity		Acquisition techniques TDI Subjects to assess and parameters to measure Septal mitral annulus early diastolic displacement velocity e’
RVOT anterograde flow		Lateral mitral annulus TDI
	Acquisition techniques PW Subjects to assess and parameters to measure RVOT anterograde flow velocity		Acquisition techniques TDI Subjects to assess and parameters to measure Lateral mitral annulus early diastolic displacement e’
Aortic anterograde flow		Lateral tricuspid annulus TDI
	Acquisition techniques PW Subjects to assess and parameters to measure AV anterograde		Acquisition techniques TDI Subjects to assess and parameters to measure Lateral tricuspid annulus systolic displacement velocity S’

Table 3

Three dimensional echocardiography views, acquisition techniques, and subjects to be assessed in patients after heart transplant"

LV 3D full volume		RV 3D full volume
Image acquisition cine (5 beats)	Acquisition techniques 3D echocardiography focused on LV Subjects to assess and parameters to measure -LAVmax, LAVmin, LAEF -LVEDV, LVESV, LVEF -LASpeak、LVGLS	Image acquisition cine (5 beats)	Acquisition techniques 3D echocardiography focused on RV Subjects to assess and parameters to measure -RAVmax, RAVmin, RAEF -RVEDV, RVESV, RVEF -RASpeak, RVFWLS

Figure 1

Two-dimensional echocardiography acquisitions in heart transplant patients using the bi-atrial technique (A) and bi-caval technique (B) respectively. LA, left atrium; LV, left ventricle; RA, right atrium; RV, right ventricle."