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Advanced Ultrasound in Diagnosis and Therapy ›› 2024, Vol. 8 ›› Issue (3): 106-115.doi: 10.37015/AUDT.2024.240017

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  • 收稿日期:2024-03-25 修回日期:2024-06-27 接受日期:2024-09-09 出版日期:2024-09-30 发布日期:2024-10-16

Ultrasound-based Dual Elastography for Evaluating the Severity of Drug-induced Liver Injury: One Step Closer to Pathology

Liyun Xue, PhDa,1, Hui Feng, MDb,1, Fankun Meng, MDc, Ying Zheng, MDc, Guangwen Cheng, PhDa, Yao Zhang, MDd, Zhiyong Yin, MDd, Jing Wu, MDe, Jiabao Zhu, MDe, Xueqi Li, MDa, Jie Yu, PhDb,*(), Ping Liang, PhDb,*(), Hong Ding, PhDa,*()   

  1. aDepartment of Ultrasound, Huashan Hospital, Fudan University, Shanghai
    bDepartment of Interventional Ultrasound, Fifth Medical Center of Chinese PLA General Hospital, Beijing
    cDepartment of Ultrasound, Beijing Youan Hospital Capital Medical University, Beijing Institute of Hepatology, Beijing
    dDepartment of Ultrasound, Beijing Ditan Hospital Capital Medical University, Beijing
    eDepartment of Ultrasound, Nantong Third Hospital Affiliated to Nantong University, Jiangsu
  • Received:2024-03-25 Revised:2024-06-27 Accepted:2024-09-09 Online:2024-09-30 Published:2024-10-16
  • Contact: *Department of Ultrasound, Huashan Hospital, Fudan University, Shanghai 200040, China (HD) e-mail: ding_hong@fudan.edu.cn (HD);Department of Interventional Ultrasound, Chinese PLA General Hospital, Beijing 100853, China (PL) e-mail: liangping301@hotmail.com(PL);Department of Interventional Ultrasound, Chinese PLA General Hospital, Beijing 100853, China (JY) e-mail: jiemi301@163.com (JY)
  • About author:First author contact:

    1 Liyun Xue and Hui Feng contributed equally to this study.

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Abstract:

Objective: Drug-induced liver injury (DILI) is one of the most challenging forms of liver disorder. We aimed to use ultrasound dual elastography, by combining strain and shear wave imaging, to noninvasively assess liver inflammation and injury severity of DILI.

Methods: 291 DILI patients were included in the prospective multicenter study and divided into training and validation cohorts. All patients received liver biopsy and dual elastography examination. Liver inflammation grading (G0-4) and fibrosis staging (F0-4) were considered as the gold standard of liver injury and G+F ≥ 5 was defined as severe liver injury. Indexes of dual elastography and serological indicators (DESI) were selected and analyzed with multivariable logistic regression to build DESI models for evaluating liver inflammation, and the C score model was built with the same method for diagnosing severe liver injury.

Results: Areas under the receiver operating characteristic curve (AUCs) of the DESI model to assess liver inflammation ≥ G2 were 0.887 and 0.868 in training and validation cohorts, respectively. AUCs of the DESI model in diagnosing ≥ G3 were 0.893 and 0.896 in the two cohorts, respectively. The C score accurately assessed severe liver injury with AUCs of 0.909 and 0.885 in two cohorts. Of the 87 patients with mild clinical severity, 10 (11.49%) had severe pathological injury, which could be identified by C score.

Conclusion: Dual elastography demonstrated high performance in diagnosing liver inflammation and identifying severe pathological liver injury of DILI, making up for the deficiency of serological indicators alone for evaluating DILI severity.

Key words: Drug-induced liver injury, Liver inflammation activity, Dual elastography, Strain elastography, Shear wave elastography

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Characteristics Training cohort (n = 232) Validation cohort (n = 59) P value
Basic characteristics
Sex (male) 70 (30.17%) 20 (33.90%) 0.580
Age (y) 46.71 ± 11.70 48.42 ± 11.43 0.332
BMI (kg/m2) 22.88 ± 3.01 23.43 ± 3.72 0.241
Laboratory tests at biopsy
Albumin (g/L) 38 (35-51) 38 (34-49) 0.874
Total bilirubin (μmol/L) 20.94 (13.6-89.1) 23.72 (12.9-202) 0.619
Alanine aminotransferase (U/L) 66.53 (35.31-247.20) 47 (35-232) 0.243
Aspartate aminotransferase (U/L) 54.50 (31-263) 53 (27-304) 0.366
Alkaline phosphatase (U/L) 106 (82-353) 105.3 (63-500) 0.160
Gamma-glutamyl transpeptidase (U/L) 99 (51-216) 92 (45-286) 0.332
The percentage of Eosinophil 1.90 (0.05-5.90) 1.30 (0.04-4.60) 0.250
Platelet count 206.50 (168-254) 210 (105-351) 0.534
International normalized ratio 1.01 (0.90-1.40) 1.00 (0.90-1.50) 0.437
Median peak values
Alanine aminotransferase (U/L) 296.50 (70-486) 263 (75-616) 0.529
Alkaline phosphatase (U/L) 321 (92-655) 306 (87-783) 0.460
Total bilirubin (μmol/L) 79.20 (15-436) 85.50 (14-397) 0.224
Median time from DILI onset to liver biopsy (d) 36 (10-92) 29 (9-80) 0.405
Injury type
Hepatocellular 113 (48.71%) 29 (49.15%) 0.933
Mixed 59 (25.43%) 16 (27.12%)
Cholestatic 60 (25.86%) 14 (23.73%)
Histopathologic results of liver biopsy
Inflammation (G) 0.247
0 12 1
1 68 19
2 74 14
3 71 22
4 6 4
Fibrosis (F) 0.804
0 125 32
1 63 14
2 44 13
3 0 0
4 0 0
Medication history
Traditional Chinese medicine and herbal and dietary supplements 91 21 0.995
Anti-infectious agents 20 5
Cardiovascular drugs 24 6
Antineoplastics 6 2
Digestive drugs 16 5
Hormone 9 3
Psychotropics 6 2
Others 23 6
Two or more classes in combination 37 9

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Item AUC (95% CI) Sensitivity (%) Specificity (%) +LR -LR P value
≥G2
Training cohort
DESI 0.887 (0.841-0.933) 82.00 87.84 6.17 0.28 /
A index 0.790 (0.728-0.853) 56.29 86.42 4.15 0.51 0.000
Vs 0.820 (0.761-0.870) 80.13 71.60 2.82 0.82 0.003
LFI 0.605 (0.535-0.672) 79.47 39.51 1.31 0.52 <0.001
Validation cohort
DESI 0.868 (0.777-0.960) 85.44 86.30 7.99 0.33 /
A index 0.757 (0.626-0.887) 94.44 56.52 2.17 0.098 0.027
Vs 0.806 (0.682-0.898) 86.11 73.91 3.3 0.19 0.158
LFI 0.667 (0.533-0.785) 30.56 95.65 7.03 0.73 0.009
≥G3
Training cohort
DESI 0.893 (0.842-0.945) 84.72 89.13 7.79 0.17 /
A index 0.815 (0.757-0.873) 75.32 72.26 2.72 0.34 0.005
Vs 0.821 (0.762-0.881) 77.92 79.35 3.77 0.28 0.014
LFI 0.622 (0.553- 0.688) 36.36 88.39 3.13 0.72 <0.001
Validation cohort
DESI 0.896 (0.808-0.984) 90.91 83.78 5.61 0.11 /
A index 0.765 (0.646-0.884) 90.91 51.35 1.87 0.18 0.020
Vs 0.809 (0.686-0.900) 81.82 75.68 3.36 0.24 0.124
LFI 0.686 (0.551-0.800) 54.55 83.78 3.36 0.54 0.006

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Clinical severity grading Pathological severity C score#
G+F<5* G+F≥5*
Mild (n = 87) 77 10 1.77 ± 0.62, P = 0.029
Moderate (n = 162) 146 16 2.25 ± 0.86, P = 0.034
Severe (n = 42) 35 7 4.30 ± 1.41

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Item G+F<5 G+F≥5 AUC (95%CI) P value* Sensitivity (%) Specificity (%) +LR -LR
Training cohort
C score 0.70 ± 0.36 4.34 ± 1.69 0.909 (0.853-0.965) / 84.62 86.96 6.49 0.18
A index 1.18 ± 0.32 1.55 ± 0.27 0.797 (0.736-0.849) 0.001 81.48 67.32 2.49 0.28
F index 1.65 ± 0.67 2.40 ± 0.57 0.755 (0.691-0.812) 0.000 96.30 45.37 1.76 0.082
Validation cohort
C score 3.22±0.53 7.11±0.86 0.885 (0.783-0.987) / 80.00 92.59 10.8 0.22
A index 1.20±0.31 1.32±0.14 0646 (0.511-0.766) 0.007 80.00 48.15 1.54 0.42
F index 1.61±0.61 1.99±0.20 0.728 (0.596-0.836) 0.047 60.00 66.67 1.80 0.60
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